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Atlas With Flow Cytometry Utilized In AML Today

Typical Immunophenotypes in AML[1,2]

CD

Antibodies

M1,M2

M3

M4,M5

M6

M7

Myeloid Lineage

CD11b

Mo1, Leu15

+/-

+/-

++

-

-

CD13

MY7, Leu-M7

++

++

++

++

+

CD14

MY4, Mo2, Leu-M3

+/-

-

++

++

-

CD15

Leu-M1

++

++

++

++

-

CD33

MY9, Leu-M9

++

++

++

++

+

T Lineage

CD2

T11, Leu-5b

+/-

+/-

+/-

-

-

CD3

T3, Leu-4

-

-

-

-

-

CD5

T1, Leu-1

-

-

-

-

-

CD7

3A1, Leu-9

+-

+/-

+/-

+/-

+

B Lineage

CD10

CALLA, J5

-

-

-

-

-

CD19

B4, Leu-12

+/-

-

+/-

-

-

CD20

B1, Leu-16

-

-

-

-

-

Erythroid Lineage

Glycophorin A

D2.10

-

-

-

++

-

CD71

T9

-

-

-

++

-

Megakaryocyte Lineage

CD41

gpIIb/IIIa

-

-

-

-

++

CD42

gpIb

-

-

-

-

++

CD61

gp/IIIa

-

-

-

-

++

Stem Cell

CD34

MY10, HPCA-1

+

-

+/-

+

+

CD38

Leu-17

+

+

+

+

+

Non-Lineage Dependent

HLA-DR

Ia, I3

++

-

++

++

+/-

Stem Cell

*The following key refers to the majority, but not all cases. These are not absolutes.

++: Strong positivity: virtually all cases

+/-: Occasional reactivity

+: Positive, but not all cases

-; Essentially negative

References

  1. Bain BJ. "Immunological, cytogenetic and other markers." In: Knowles Z, ed. Leukemia Diangosis- A Guide to the FAB Classification. Philadelphia, PA: JB Lippincott Co; 1990:61-88.

  2. Carey, JL, Hanson, CA. "Flow cytometric analysis of leukemia and lymphoma." In: Kerne DF, Hanson, CA, Hurtubise, PF, eds. Flow Cytometry and Clinical Diagnosis. Chicago, IL; ASCP Press; 1993:197-308.

© Copyright 1995 Pharmacia, Inc.
October 1995

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