[MOL] Fine needle aspiration may shed breast cells into peripheral blood [01216] Medicine On Line


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[MOL] Fine needle aspiration may shed breast cells into peripheral blood...



A DGReview of :"Fine Needle Aspiration May Shed Breast Cells into Peripheral Blood as Determined by RT-PCR"
Oncology

10/25/2000
By Veronica Rose


A recent report suggests that haematogenous dissemination of breast cells may be caused by fine needle aspiration to beast tumour

Researchers from Hong Kong evaluated the impact of fine needle aspiration (FNA) on breast cell shedding into peripheral blood. They applied reverse- transcriptase chain reaction (RT-PCR) assay targeted, against cytokeratin 19 (CK19) cytokeratin 20 (CK20) and the -subunit of human chorionic gonadotropin (-hCG) messenger RNAs.

The sensitivity of this assay was based on the different degree of admix of MCF-7 breast cancer cell line with HL-60 leukemic cell line. For blood samples from 24 cases with benign breast disease and 20 cases with malignant diseases, 5 mL of peripheral blood was drawn prior to and within 10 minutes following puncture.

Total RNA was extracted from the peripheral blood mononuclear cells. -actin was used to assess the quality of cRNA. Reverse-transcriptase chain reaction products were run in ethidium bromide gel and observed under ultraviolet. Reverse-transcriptase chain reaction products for -human chorionic gonadotropin were digested with Sty 1 endonucleatase to confirm the specificity.

Reverse transcriptase-chain reaction assay sensitivity was 1 MCF-7 cell in 10 (to the power of 5) HL-60 cells for cytokeratin 19 and cytokeratin 20, and 1 in 10 (to the power of 6) for B-hcg. The 24 benign cases, none of the pre-fine needle aspiration samples proved positive to CK-20 and B-hcg. Three cases were positive for CK19. Relative to the 20 malignant cases, one pre-FNA sample was positive for all three markers and two other samples proved positive for CK19.

Following aspiration, three of 21 benign cases and one of 17 malignant cases with pre-FNA-negative signals became positive for CK19, and three of 19 malignant cases with pre-FNA-negative signals were positive for CK20 and -hcg. Of six pre-FNA-positive cases, all remained positive for the respective marker.

 
 
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