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Do you know whether the cell line you are using in your research is authentic?
For example, a cell line
which is believed to be of human breast cancer may actually be the cell line of
a human prostate cancer, mouse or Syrian hamster.
Cell line contamination is
not uncommon incident in a laboratory handling more than one cell line. Cell
line contamination may also be caused by the transformation of host cells by the
injected human tumor cells. Human tumors injected into nude murine animals may
transform host cells and the tumor growing in the host animal may be a mixture
of human and murine cells or purely of murine origin (Oncol Res 9:433-438, 1997;
Br J Cancer 76:1134-1138, 1997). Inter- or intraspecies cell line contamination
can easily be identified by conventional and molecular cytogenetics. It can save
valuable time, effort, and precious research funds and prevent investigators
from innocently publishing erroneous molecular data and misleading conclusions.
Click on the
images below for full size and description:
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| Human (injected).jpg A G-banded karyotype of human cell line before injection into nude mice |
Mouse (harvested).jpg
A karyotype of cells harvested from a nude mice in which human prostate cancer cells were injected |
Fish1.jpg FISH image showing no human DNA in a mouse cell line harvested from a nude mice in which human cancer cells were injected |
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